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mfd [2017-05-24 16:10:57]
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mfd [2017-05-24 16:10:57]

transcription-repair coupling factor, eliminates genetic damage from transcriptionally active genes during sporulation, required for increased mutagenesis of lagging strand genes
Locus
BSU00550
Isoelectric point
5.37
Molecular weight
133.59 kDa
Protein length
1177 aa Sequence Blast
Gene length
3531 bp Sequence Blast
Function
promotes strand-specific DNA repair by displacing
Product
transcription-repair coupling factor
Essential
no
E.C.
3.6.4.-
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
60,430 → 63,963

Phenotypes of a mutant

  • in an mfd knock-out, the cell's ability to accumulate adaptive mutations in stationary phase is depressed PubMed
  • strongly reduced stationary phase mutagenesis PubMed
  • increased UV-induced mutagenesis via PolY1/ PolY2-mediated translesion synthesis PubMed
  • the mutation suppresses the mucoid phenotype of motA or motB mutants PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • promotes strand-specific DNA repair by displacing RNA polymerase stalled at a nucleotide lesion and directing the (A)BC excinuclease to the RNA damage site
  • is required for roadblock transcription repression by transcription factors with binding sites downstream of the promoter (as for CcpA PubMed and CodY PubMed)
  • required for the processing of genetic damage during sporulation PubMed
  • Paralogous protein(s)

    Structure

  • 2EYQ (Mfd from E. coli) PubMed
  • 3MLQ (RNA polymerase interacting domain of Thermus thermophilus Mfd with the Thermus aquaticus RpoB beta1 domain) PubMed
  • Expression and Regulation

    Operons

    Biological materials

    Mutant

  • GP1167 (del ermC), available in Jörg Stülke's lab PubMed
  • BKE00550 (mfd::erm, available in the BGSC, in Fabian Commichau's, and in Jörg Stülke's labs)
  • GFP fusion

  • GP1510 (spc, based on pGP1870, pGP1389-derivative ), available in Jörg Stülke's lab
  • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab
  • Antibody

  • **
  • References

    Reviews

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    Original publications

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